ABSTRACT
Diseases caused by dengue, s and fly fever and hanta viruses pose a major health risk in many countries. We determined the threat of these arboviral infections through a serologic using enzyme linked immunosorbent assay [ELISA] based tests. Hantavirus-specific antibodies were also detected using immunofluorescence. Of 499 samples tested for dengue virus IgG antibodies l4% were as positive for dengue positive by all the ELISA tests. Among the 42 showing strong IgG reactivity, only 1 was positive for dengue virus IgM antibodies. All samples tested for IgG antibodies to s and fly fever virus were negative. Hantavirus antibodies were detected in 11% of the 46 samples from high-risk individuals. The low prevalences suggest that at present these infections are not a serious problem in Kuwait
Subject(s)
Adolescent , Adult , Child , Humans , Infant , Middle Aged , Age Distribution , Antibodies, Viral/blood , Arboviruses/immunology , Bunyaviridae/immunology , Dengue/epidemiology , Enzyme-Linked Immunosorbent Assay , Orthohantavirus/immunology , Hantavirus Infections/epidemiology , Phlebotomus Fever/bloodABSTRACT
Wanowrie virus (WAN) is an ungrouped arbovirus having morphogenetic characters in common with Bunyavirus. It was isolated for the first time from ticks, Hyalomma marginatum isacci (6700 strain) and subsequently from a human case (68923 strain). In the absence of any reports on the prevalence of this virus amongst humans in India, 600 human sera were tested in the Complement Fixation (CF) test against both the strains of WAN virus. One hundred human sera were randomly selected from six different states, viz Jammu and Kashmir, Rajasthan, Orissa, Maharashtra, Karnataka and Tamil Nadu. CF antibodies were detected in 77 sera (12.8%) against the 6700 antigen while 91 sera (15.1%) possessed antibodies against the 68923 antigen; 60 sera were found to be reactive against both the antigens, with almost identical titres. There was no statistical difference in the distribution of CF reactive sera with respect to age, gender and geographic locations. All the CF reactive sera were found to be negative when tested in the neutralization test against the respective virus strains. The CF antibodies detected in these sera may have been produced during exposure to an antigenically closely related virus yet to be identified.
Subject(s)
Adolescent , Adult , Aged , Animals , Antibodies, Viral/blood , Antigens, Viral/immunology , Bunyaviridae/immunology , Bunyaviridae Infections/epidemiology , Child , Child, Preschool , Complement Fixation Tests , Female , Humans , India/epidemiology , Infant , Infant, Newborn , Male , Middle Aged , Retrospective StudiesABSTRACT
A caracterizaçäo antigênica do vírus Jatobal (BeAn 423380) foi efetuada utilizando uma técnica de ELISA para deteccäo de anticorpos que utiliza culturas celulares infectadas como antígeno e um micro teste de neutralizaçäo para vírus que utiliza o método imunoenzimático como auxiliar para a leitura dos resultados (NT-EIA). O vírus Jatobal foi caracterizado como um Bunyaviridae, gênero Bunyavirus, pertencente ao sorogrupo Simbu. A técnica de ELISA, utilizando culturas celulares infectadas como antígeno, trata-se de método sensível e confiável na identificaçäo de agentes virais, possuindo muitas vantagens sobre ELISA convencionais e outros testes: elimina a preparaçäo laboriosa de antígenos para o revestimento em fase sólida; permite que se teste de forma mais rápida e fácil que por CF, HAI e neutralizaçäo por reduçäo de plaques um grande número de antisoros de vírus. ELISA e NT-EIA podem ser utilizados para a classificaçäo de vírus que näo produzem efeito citopático e podem ser aplicáveis na identificaçäo de vírus que crescem em células oriundas de mosquitos